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GC-MS Analysis of Phytocompounds of Leaf and Stem of Marsilea quadrifolia (L.). England: Handbook and Directory of Comercial Laboratories, Easter Press. Jurnal Kesehatan Masyarakat, 7(1), 26-31. Asian Journal of Pharmaceutical Science & Technology, 5(2), 64-69.ĭiana, F, M. GC-MS Evaluation of Bioactive Molecules from the Methanolic Leaf Extract of Azadirachta indica. Mini-Reviews in Mdicinal Chemistry, 13(2), 1-7.ĭineshkumar, G.
An Overview of the Modulatory Effect of Oleic Acid in Health and Disease. European Journal Pharmacology, 667, 410-418.Ĭampos, H. Antidiabetic Activity of γ-Sitosterol Isolated from Lippia nodiflora L. International Journal of Pharmacy and Pharmaceutical Science, 10(9), 68-72.īalamurugan, R., Duraipandiyan, V., & Ignacimuthu, S. In Vitro Callus Induction and Comparative GC-MS Analysis of Methanolic Extract of Callus and Leaf Samples of Ampelocissus latifolia (Roxb) Planch. Minyak Atsiri Tumbuhan Tropika Indonesia. Var Nigra, secondary metabolite compoundsĪgusta, A.
GCMS analysis showed that secondary metabolites with the highest percentage in each treatment were gamma sitosterol compound (14.88%) in treatment I1.0K1.0 14-Beta H Pregna compound (15.94%) in I1.0K1.5 treatment and beta-d-glucopyranoside compound (15.54%) in I0.5B0.5 treatment and beta-d-glucopyranoside compound (5.63%) in I1.0 B1.5 treatment. Simplisia of black betel’s callus was macerated using methanol solvent and identified using Gas Chromatography Mass Spectra (GCMS). The callus was maintained for eight weeks. The explant of black betel’s leaf was cultured on Murashige and Skoog medium with four combination treatments (I1.0K1.0 I1.0K1.5 I1B1.5 I0.5B0.5). This study was aims to determine the effect of variations in growth regulator Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), and Kinetin on secondary metabolite compounds contained in black betel callus. Var Nigra (black betel) is one type of medicinal plant that has the potential to be developed by tissue culture method. Berdasarkan analisis GCMS menunjukkan bahwa senyawa metabolit sekunder dengan presentase tertinggi terdapat pada masing- masing perlakuan yaitu senyawa gamma sitosterol (14,88%) pada perlakuan I1,0K1,0 senyawa 14-Beta H Pregna (15,94%) pada perlakuan I1,0K1,5 serta senyawa beta-d-glucopyranoside (15,54%) pada perlakuan I0,5B0,5 dan senyawa beta-d-glucopyranoside (5,63%) pada perlakuan I1,0 B1,5. Simplisia kalus sirih hitam di maserasi menggunakan pelarut metanol dan diidentifikasi menggunakan Gas Chromatography Mass Spectra (GCMS). Eksplan daun dari sirih hitam ditanam pada medium Murashige dan Skoog dengan 4 perlakuan kombinasi (I1,0K1,0 I1,0K1,5 I1B1,5 I0,5B0,5). Penelitian ini bertujuan untuk mengetahui pengaruh variasi zat pengatur tumbuh Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin terhadap senyawa metabolit sekunder yang terdapat kalus sirih hitam. var Nigra (sirih hitam) merupakan salah satu jenis tanaman obat yang berpotensi untuk dikembangkan dengan metode kultur jaringan.
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